Desiccation and storage of Caenorhabditis elegans for use in aquaculture

Autores/as

  • M Sauci Jimenez Departmento de Biología Molecular e Ingeniería Bioquímica/CABD-UPO, Ctra. Utrera Km 1. 41013 - Sevilla
  • M.J Muñoz Ruiz Departmento de Biología Molecular e Ingeniería Bioquímica/CABD-UPO, Ctra. Utrera Km 1. 41013 - Sevilla
  • A.M. Brokate Llanos Departmento de Biología Molecular e Ingeniería Bioquímica/CABD-UPO, Ctra. Utrera Km 1. 41013 - Sevilla

Palabras clave:

Aquaculture; Desiccation; storage; Caenorhabditis elegans; salt

Resumen

Aquaculture is a thriving sector, although the main issues it faces at the moment is the feeding of fish at an early stage of development. The most utilised specie for that is Artemia. Nematodes could replace Artemia in larviculture thanks to its rapid development by self-fertilization and its ability to be produced in a massive way fed with bacteria. Therefore, it would reduce the price of the raw.
The use of nematodes for feeding fish larvae in aquaculture has required an adaptation to different salt concentrations in liquid medium. There is also necessary to develop an efficient protocol for the desiccation and storage of nematodes at different larval stages, this would allow the product to be transported to fish farms worldwide.
We are developing desecating protocols for different conditions such as time, temperature and relative humidity. In this way we will be able to determine which of them is the most effective

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Citas

McClanahan, P. D., McCloskey, R. J., Hing, M. N. T., Raizen, D. M., & Fang-Yen, C. (2020). Dehydrated caenorhabditis elegans stocks are resistant to multiple freeze-thaw cycles. G3: Genes, Genomes, Genetics, 10(12), 4505–4512. https://doi.org/10.1534/g3.120.401825

Erkut, C., Vasilj, A., Boland, S., Habermann, B., Shevchenko, A., & Kurzchalia, T. V. (2013). Molecular strategies of the Caenorhabditis elegans dauer larva to survive extreme desiccation. PLoS ONE, 8(12), 1–19. https://doi.org/10.1371/journal.pone.0082473

Stiernagle, T. (2006). Maintenance of C. elegans. WormBook : The Online Review of C. Elegans Biology, 6(2000), 1–11. https://doi.org/10.1895/wormbook.1.101.1

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Publicado

2021-03-18

Cómo citar

(1)
Sauci Jimenez, M.; Muñoz Ruiz, . M.; Brokate Llanos, A. Desiccation and Storage of Caenorhabditis Elegans for Use in Aquaculture. Bs 2021.

Número

Núm. 10 (2021)

Sección

Pósteres

Licencia

Derechos de autor 2021 Biosaia: Revista de los másteres de Biotecnología Sanitaria y Biotecnología Ambiental, Industrial y Alimentaria

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.