Role of aak-2 in neuroprotective action in Alzheimer's and Parkinson's disease models in C. elegans.
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aak-2, C. elegans, neurodegenerative diseasesResumen
Caenorhabditis elegans is an important model organism as it shares genetic and physiological similarities with humans.. Age-related diseases, such as Alzheimer's or Parkinson's, are caused by the progressive decline of proteostasis during the aging, characterized by the appearance of protein aggregates, which can cause cell function failure in addition to cell death (1).
An increase in sulfated steroid hormones has been shown to extend lifespan and ameliorate diseases caused by aging. For this purpose, sul-2 mutants were generated. Sul-2 is a sulfatase of steroid hormones. When the sulfatase gene is deleted, the ability to remove sulfate from hormones is lost and there is an increase in sulfated steroid hormones in the organism, and a reduction in symptoms of neurodegenerative diseases and an increase in longevity. Treatment with the drug STX64, a specific inhibitor of the steroid sulfatase enzyme, in C. elegans and in murine models has been shown to produce the same beneficial effects (2).
Recently in our laboratory, results obtained in a RNA seq show that sul-2 mutants share expression patterns with AMPK activation mutants. There are two AMPKα subunit homologs in C. elegans, aak-1 and aak-2. aak-2 is the homologous gene of AMPK in humans, a cellular fuel sensor that regulates cellular energy homeostasis and functions in stress resistance and to extend lifespan (3). The results of a research show that AMPK activation also has neuroprotective effects in Huntington's disease, its activation can preserve striatal neurons to combat the consequences of toxicity in murine models and protects C. elegans neurons from the dysfunction induced by human exon-1 huntingtin expression (4).
To know if aak-2 has a role in the neuroprotective effects of sul-2 mutants, we have constructed aak-2 mutants in Alzheimer's and Parkinson's models to check the effects of aak-2. The different strains are subjected to different assays, Alzheimer’s model show a paralysis phenotype when they are shifted to 25ºC and Parkinson’s models show slow movement in buffer, so we perform thrashing assays. In subsequent assays, an aak-2;sul-2 double mutant will be generated to test whether the neuroprotective action of sul-2 mutants is reversed. At the same time, we have started assays with STX64, another way of looking at the consequences of sul-2 deletion.
The aim of the project is to understand the role of aak-2 in proteostasis and neurodegenerative diseases and in this way provide a better understanding of the mechanism of action of the drug STX64.
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Labbadia J and Morimoto R.I. (2015) The biology of proteostasis in aging and disease. Annu Rev Biochem. 84: 435-64
https://doi.org/10.1146/annurev-biochem-060614-033955
Pérez-Jiménez, M.M.. et al. (2021) Steroid hormones sulfatase inactivation extends lifespan and ameliorates age-related diseases. Nat Commun 12, 49
https://doi.org/10.1038/s41467-020-20269-y
Apfeld, J. et al. (2004) The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. Genes & Dev. 18: 3004-3009
https://doi.org/10.1101/gad.1255404
Vázquez-Manrique RP et al. (2016). AMPK activation protects from neuronal dysfunction and vulnerability across nematode, cellular and mouse models of Huntington's disease. Hum Mol Genet.25(6):1043-58
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Derechos de autor 2023 Biosaia: Revista de los másteres de Biotecnología Sanitaria y Biotecnología Ambiental, Industrial y Alimentaria
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